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recombinant human mouse rat activin a  (R&D Systems)


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    R&D Systems recombinant human mouse rat activin a
    Recombinant Human Mouse Rat Activin A, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 589 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+activin+a/Recombinant+Human%2FMouse%2FRat+Activin+A+Protein/pmc13120857-25-22-27
    Average 96 stars, based on 589 article reviews
    recombinant human mouse rat activin a - by Bioz Stars, 2026-09
    96/100 stars

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    Cell Culture:

    Article Title: Glycan-binding properties of SARS-CoV-2 spike proteins: interactions with aminoglycoside antibiotics.
    Article Snippet: The differentiation of the human lung organoids was performed in serum-free differentiation (SFD) medium, composed of DMEM/F12 (3:1) (FUJIFILM Wako Pure Chemical and Thermo Fisher Scientific) supplemented with N2 (FUJIFILM Wako Pure Chemical), B-27 Supplement Minus Vitamin A (Thermo Fisher Scientific), 50 μg/mL ascorbic acid (STEMCELL Technologies), 1 × GlutaMAX (Thermo Fisher Scientific), 1% monothioglycerol (FUJIFILM Wako Pure Chemical), 0.05% BSA (Sigma-Aldrich), and 100 U/mL penicillin, 100 μg/mL streptomycin (P/S; Thermo Fisher Scientific). .. During days 0–1 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical) ACCEPTED MANUSCRIPT ARTICLE IN PRESS AR TIC LE IN PR ES S and 100 ng/mL recombinant Activin A (R&D Systems). .. During days 1–3 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical), 100 ng/mL recombinant Activin A (R&D Systems) and 1% FBS.

    Article Title: Dual inhibition of intercellular adhesion molecule-1 and nucleolin reduces RSV infection efficiency in human respiratory organoids
    Article Snippet: The differentiation of the respiratory 322 organoids was performed in serum-free differentiation (SFD) medium, composed 323 of DMEM/F12 (3:1) (Cat# 044-29765, FUJIFILM Wako Pure Chemical and Cat# 324 11320033, Thermo Fisher Scientific) supplemented with N2 (Cat# 141-08941, 325 FUJIFILM Wako Pure Chemical), B-27 Supplement Minus Vitamin A (Cat# 326 12587001, Thermo Fisher Scientific), ascorbic acid (50 μg/mL, Cat# ST-72132, 327 STEMCELL Technologies), 1× GlutaMAX (Cat# 35050-061, Thermo Fisher 328 Scientific), 0.5 mmol/mL monothioglycerol (Cat# 195-15791, FUJIFILM Wako 329 Pure Chemical), 0.05% bovine serum albumin (Cat# 820024, Sigma-Aldrich), and 330 1× penicillin/streptomycin. .. During days 0-1 of differentiation, cells were cultured 331 Jo urn al Pr eroo f 11 with SFD medium supplemented with 10 μM Y-27632 (Cat# 034-24024, 332 FUJIFILM Wako Pure Chemical) and 100 ng/mL recombinant Activin A (Cat# 333 338-AC-01M, R&D Systems). .. During days 1–3 of differentiation, cells were 334 cultured with SFD medium supplemented with 10 μM Y-27632 (Cat# 034-24024, 335 FUJIFILM Wako Pure Chemical), 100 ng/mL recombinant Activin A (Cat# 338-336 AC-01M, R&D Systems) and 1% FBS.

    Article Title: Glycan-binding properties of SARS-CoV-2 spike proteins: interactions with aminoglycoside antibiotics
    Article Snippet: During days 0–1 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical) and 100 ng/mL recombinant Activin A (R&D Systems). .. During days 1–3 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical), 100 ng/mL recombinant Activin A (R&D Systems) and 1% FBS. .. Between days 3–5 of differentiation, cells were cultured in SFD medium supplemented with 1.5 μM Dorsomorphin dihydrochloride (FUJIFILM Wako Pure Chemical) and 10 μM SB431542 (FUJIFILM Wako Pure Chemical) for 24 h, and then SFD medium supplemented with 10 μM SB431542 and 1 μM Stemolecule Wnt inhibitor IWP2 (FUJIFILM Wako Pure Chemical) for another 24 h. During days 5–12 of differentiation, cells were cultured with SFD medium supplemented with 3 μM CHIR99021 (FUJIFILM Wako Pure Chemical), 10 ng/mL human FGF10 (PeproTech, NJ, USA), 10 ng/mL human fibroblast growth factor (FGF) 10 (PeproTech), 10 ng/mL human bone morphogenetic protein (BMP), 20 ng/mL human epidermal growth factor (PeproTech), and all-trans retinoic acid (ATRA; Sigma-Aldrich).

    Article Title: Dual inhibition of intercellular adhesion molecule-1 and nucleolin reduces RSV infection efficiency in human respiratory organoids
    Article Snippet: During days 0-1 of differentiation, cells were cultured 331 Jo urn al Pr eroo f 11 with SFD medium supplemented with 10 μM Y-27632 (Cat# 034-24024, 332 FUJIFILM Wako Pure Chemical) and 100 ng/mL recombinant Activin A (Cat# 333 338-AC-01M, R&D Systems). .. During days 1–3 of differentiation, cells were 334 cultured with SFD medium supplemented with 10 μM Y-27632 (Cat# 034-24024, 335 FUJIFILM Wako Pure Chemical), 100 ng/mL recombinant Activin A (Cat# 338-336 AC-01M, R&D Systems) and 1% FBS. .. During days 3–5, cells were cultured in 337 SFD medium supplemented with 1.5 μM dorsomorphin dihydrochloride (Cat# 338 047-33763, FUJIFILM Wako Pure Chemical) and 10 μM SB431542 (Cat# 037-339 24293, FUJIFILM Wako Pure Chemical) for 24 h, followed by SFD medium 340 supplemented with 10 μM SB431542 and 1 μM IWP2 (Cat# 04-0034, 341 Stemolecule) for an additional 24 h. During days 5–12, cells were cultured with 342 SFD medium supplemented with 3 μM CHIR99021 (Cat# 034-23103, FUJIFILM 343 Wako Pure Chemical), 10 ng/mL human FGF10 (Cat# AF-100-26, PeproTech), 344 10 ng/mL human FGF7 (Cat# AF-100-19, PeproTech), 10 ng/mL human BMP4 345 (Cat# 120-05ET, PeproTech), 20 ng/mL human EGF (Cat# AF-100-15, 346 PeproTech), and 50 nM all-trans retinoic acid (ATRA, Cat# R2625, Sigma-347 Aldrich).

    Article Title: Glycan-binding properties of SARS-CoV-2 spike proteins: interactions with aminoglycoside antibiotics
    Article Snippet: The differentiation of the human lung organoids was performed in serum-free differentiation (SFD) medium, composed of DMEM/F12 (3:1) (FUJIFILM Wako Pure Chemical and Thermo Fisher Scientific) supplemented with N2 (FUJIFILM Wako Pure Chemical), B-27 Supplement Minus Vitamin A (Thermo Fisher Scientific), 50 μg/mL ascorbic acid (STEMCELL Technologies), 1 × GlutaMAX (Thermo Fisher Scientific), 1% monothioglycerol (FUJIFILM Wako Pure Chemical), 0.05% BSA (Sigma-Aldrich), and 100 U/mL penicillin, 100 μg/mL streptomycin (P/S; Thermo Fisher Scientific). .. During days 0–1 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical) and 100 ng/mL recombinant Activin A (R&D Systems). .. During days 1–3 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical), 100 ng/mL recombinant Activin A (R&D Systems) and 1% FBS.

    Article Title: Glycan-binding properties of SARS-CoV-2 spike proteins: interactions with aminoglycoside antibiotics.
    Article Snippet: During days 0–1 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical) ACCEPTED MANUSCRIPT ARTICLE IN PRESS AR TIC LE IN PR ES S and 100 ng/mL recombinant Activin A (R&D Systems). .. During days 1–3 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical), 100 ng/mL recombinant Activin A (R&D Systems) and 1% FBS. .. Between days 3–5 of differentiation, cells were cultured in SFD medium supplemented with 1.5 μM Dorsomorphin dihydrochloride (FUJIFILM Wako Pure Chemical) and 10 μM SB431542 (FUJIFILM Wako Pure Chemical) for 24 h, and then SFD medium supplemented with 10 μM SB431542 and 1 μM Stemolecule Wnt inhibitor IWP2 (FUJIFILM Wako Pure Chemical) for another 24 h. During days 5–12 of differentiation, cells were cultured with SFD medium supplemented with 3 μM CHIR99021 (FUJIFILM Wako Pure Chemical), 10 ng/mL human FGF10 (PeproTech, NJ, USA), 10 ng/mL human fibroblast growth factor (FGF) 10 (PeproTech), 10 ng/mL human bone morphogenetic protein (BMP), 20 ng/mL human epidermal growth factor (PeproTech), and all-trans retinoic acid (ATRA; Sigma-Aldrich).

    Article Title: Glycan-binding properties of SARS-CoV-2 spike proteins: interactions with aminoglycoside antibiotics.
    Article Snippet: .. During days 0–1 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical) AR TIC LE IN PR ES S and 100 ng/mL recombinant Activin A (R&D Systems). ..

    Recombinant:

    Article Title: Glycan-binding properties of SARS-CoV-2 spike proteins: interactions with aminoglycoside antibiotics.
    Article Snippet: The differentiation of the human lung organoids was performed in serum-free differentiation (SFD) medium, composed of DMEM/F12 (3:1) (FUJIFILM Wako Pure Chemical and Thermo Fisher Scientific) supplemented with N2 (FUJIFILM Wako Pure Chemical), B-27 Supplement Minus Vitamin A (Thermo Fisher Scientific), 50 μg/mL ascorbic acid (STEMCELL Technologies), 1 × GlutaMAX (Thermo Fisher Scientific), 1% monothioglycerol (FUJIFILM Wako Pure Chemical), 0.05% BSA (Sigma-Aldrich), and 100 U/mL penicillin, 100 μg/mL streptomycin (P/S; Thermo Fisher Scientific). .. During days 0–1 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical) ACCEPTED MANUSCRIPT ARTICLE IN PRESS AR TIC LE IN PR ES S and 100 ng/mL recombinant Activin A (R&D Systems). .. During days 1–3 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical), 100 ng/mL recombinant Activin A (R&D Systems) and 1% FBS.

    Article Title: Dual inhibition of intercellular adhesion molecule-1 and nucleolin reduces RSV infection efficiency in human respiratory organoids
    Article Snippet: The differentiation of the respiratory 322 organoids was performed in serum-free differentiation (SFD) medium, composed 323 of DMEM/F12 (3:1) (Cat# 044-29765, FUJIFILM Wako Pure Chemical and Cat# 324 11320033, Thermo Fisher Scientific) supplemented with N2 (Cat# 141-08941, 325 FUJIFILM Wako Pure Chemical), B-27 Supplement Minus Vitamin A (Cat# 326 12587001, Thermo Fisher Scientific), ascorbic acid (50 μg/mL, Cat# ST-72132, 327 STEMCELL Technologies), 1× GlutaMAX (Cat# 35050-061, Thermo Fisher 328 Scientific), 0.5 mmol/mL monothioglycerol (Cat# 195-15791, FUJIFILM Wako 329 Pure Chemical), 0.05% bovine serum albumin (Cat# 820024, Sigma-Aldrich), and 330 1× penicillin/streptomycin. .. During days 0-1 of differentiation, cells were cultured 331 Jo urn al Pr eroo f 11 with SFD medium supplemented with 10 μM Y-27632 (Cat# 034-24024, 332 FUJIFILM Wako Pure Chemical) and 100 ng/mL recombinant Activin A (Cat# 333 338-AC-01M, R&D Systems). .. During days 1–3 of differentiation, cells were 334 cultured with SFD medium supplemented with 10 μM Y-27632 (Cat# 034-24024, 335 FUJIFILM Wako Pure Chemical), 100 ng/mL recombinant Activin A (Cat# 338-336 AC-01M, R&D Systems) and 1% FBS.

    Article Title: Glycan-binding properties of SARS-CoV-2 spike proteins: interactions with aminoglycoside antibiotics
    Article Snippet: During days 0–1 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical) and 100 ng/mL recombinant Activin A (R&D Systems). .. During days 1–3 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical), 100 ng/mL recombinant Activin A (R&D Systems) and 1% FBS. .. Between days 3–5 of differentiation, cells were cultured in SFD medium supplemented with 1.5 μM Dorsomorphin dihydrochloride (FUJIFILM Wako Pure Chemical) and 10 μM SB431542 (FUJIFILM Wako Pure Chemical) for 24 h, and then SFD medium supplemented with 10 μM SB431542 and 1 μM Stemolecule Wnt inhibitor IWP2 (FUJIFILM Wako Pure Chemical) for another 24 h. During days 5–12 of differentiation, cells were cultured with SFD medium supplemented with 3 μM CHIR99021 (FUJIFILM Wako Pure Chemical), 10 ng/mL human FGF10 (PeproTech, NJ, USA), 10 ng/mL human fibroblast growth factor (FGF) 10 (PeproTech), 10 ng/mL human bone morphogenetic protein (BMP), 20 ng/mL human epidermal growth factor (PeproTech), and all-trans retinoic acid (ATRA; Sigma-Aldrich).

    Article Title: Dual inhibition of intercellular adhesion molecule-1 and nucleolin reduces RSV infection efficiency in human respiratory organoids
    Article Snippet: During days 0-1 of differentiation, cells were cultured 331 Jo urn al Pr eroo f 11 with SFD medium supplemented with 10 μM Y-27632 (Cat# 034-24024, 332 FUJIFILM Wako Pure Chemical) and 100 ng/mL recombinant Activin A (Cat# 333 338-AC-01M, R&D Systems). .. During days 1–3 of differentiation, cells were 334 cultured with SFD medium supplemented with 10 μM Y-27632 (Cat# 034-24024, 335 FUJIFILM Wako Pure Chemical), 100 ng/mL recombinant Activin A (Cat# 338-336 AC-01M, R&D Systems) and 1% FBS. .. During days 3–5, cells were cultured in 337 SFD medium supplemented with 1.5 μM dorsomorphin dihydrochloride (Cat# 338 047-33763, FUJIFILM Wako Pure Chemical) and 10 μM SB431542 (Cat# 037-339 24293, FUJIFILM Wako Pure Chemical) for 24 h, followed by SFD medium 340 supplemented with 10 μM SB431542 and 1 μM IWP2 (Cat# 04-0034, 341 Stemolecule) for an additional 24 h. During days 5–12, cells were cultured with 342 SFD medium supplemented with 3 μM CHIR99021 (Cat# 034-23103, FUJIFILM 343 Wako Pure Chemical), 10 ng/mL human FGF10 (Cat# AF-100-26, PeproTech), 344 10 ng/mL human FGF7 (Cat# AF-100-19, PeproTech), 10 ng/mL human BMP4 345 (Cat# 120-05ET, PeproTech), 20 ng/mL human EGF (Cat# AF-100-15, 346 PeproTech), and 50 nM all-trans retinoic acid (ATRA, Cat# R2625, Sigma-347 Aldrich).

    Article Title: Glycan-binding properties of SARS-CoV-2 spike proteins: interactions with aminoglycoside antibiotics
    Article Snippet: The differentiation of the human lung organoids was performed in serum-free differentiation (SFD) medium, composed of DMEM/F12 (3:1) (FUJIFILM Wako Pure Chemical and Thermo Fisher Scientific) supplemented with N2 (FUJIFILM Wako Pure Chemical), B-27 Supplement Minus Vitamin A (Thermo Fisher Scientific), 50 μg/mL ascorbic acid (STEMCELL Technologies), 1 × GlutaMAX (Thermo Fisher Scientific), 1% monothioglycerol (FUJIFILM Wako Pure Chemical), 0.05% BSA (Sigma-Aldrich), and 100 U/mL penicillin, 100 μg/mL streptomycin (P/S; Thermo Fisher Scientific). .. During days 0–1 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical) and 100 ng/mL recombinant Activin A (R&D Systems). .. During days 1–3 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical), 100 ng/mL recombinant Activin A (R&D Systems) and 1% FBS.

    Article Title: Glycan-binding properties of SARS-CoV-2 spike proteins: interactions with aminoglycoside antibiotics.
    Article Snippet: During days 0–1 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical) ACCEPTED MANUSCRIPT ARTICLE IN PRESS AR TIC LE IN PR ES S and 100 ng/mL recombinant Activin A (R&D Systems). .. During days 1–3 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical), 100 ng/mL recombinant Activin A (R&D Systems) and 1% FBS. .. Between days 3–5 of differentiation, cells were cultured in SFD medium supplemented with 1.5 μM Dorsomorphin dihydrochloride (FUJIFILM Wako Pure Chemical) and 10 μM SB431542 (FUJIFILM Wako Pure Chemical) for 24 h, and then SFD medium supplemented with 10 μM SB431542 and 1 μM Stemolecule Wnt inhibitor IWP2 (FUJIFILM Wako Pure Chemical) for another 24 h. During days 5–12 of differentiation, cells were cultured with SFD medium supplemented with 3 μM CHIR99021 (FUJIFILM Wako Pure Chemical), 10 ng/mL human FGF10 (PeproTech, NJ, USA), 10 ng/mL human fibroblast growth factor (FGF) 10 (PeproTech), 10 ng/mL human bone morphogenetic protein (BMP), 20 ng/mL human epidermal growth factor (PeproTech), and all-trans retinoic acid (ATRA; Sigma-Aldrich).

    Article Title: CRISPR/Cas9-mediated gene correction of Wilson disease H1069Q point mutation in patient-specific induced pluripotent stem cells.
    Article Snippet: .. Briefly, cells were incubated in DMEM/F12 (Gibco, Thermo Fisher Scientific) with increasing KnockOut SR Xenofree medium (KSR; Gibco, Thermo Fisher Scientific) concentrations, enriched with 100 ng/μl recombinant Activin-A (R&D Systems, Minneapolis, MN, USA), 100 ng/μl FGF2 (PeproTech, Thermo Fisher Scientific), and 10 μg/ml recombinant human Wnt-3a (R&D Systems) for 3 days (day 1 to 3). .. For the next 8 days (days 4 to 11), cells were cultivated in DMEM/ F12 supplemented with 10% KSR, 1 mM NEAA, 1 mM L-glutamine (Life Technologies), 1% dimethyl sulfoxide (PanReacAppliChem, Darmstadt, Germany), and 100 ng/μl hepatocyte growth factor (PeproTech, Thermo Fisher Scientific).

    Article Title: Glycan-binding properties of SARS-CoV-2 spike proteins: interactions with aminoglycoside antibiotics.
    Article Snippet: .. During days 0–1 of differentiation, cells were cultured with SFD medium supplemented with 10 μM Y-27632 (FUJIFILM Wako Pure Chemical) AR TIC LE IN PR ES S and 100 ng/mL recombinant Activin A (R&D Systems). ..

    Incubation:

    Article Title: CRISPR/Cas9-mediated gene correction of Wilson disease H1069Q point mutation in patient-specific induced pluripotent stem cells.
    Article Snippet: .. Briefly, cells were incubated in DMEM/F12 (Gibco, Thermo Fisher Scientific) with increasing KnockOut SR Xenofree medium (KSR; Gibco, Thermo Fisher Scientific) concentrations, enriched with 100 ng/μl recombinant Activin-A (R&D Systems, Minneapolis, MN, USA), 100 ng/μl FGF2 (PeproTech, Thermo Fisher Scientific), and 10 μg/ml recombinant human Wnt-3a (R&D Systems) for 3 days (day 1 to 3). .. For the next 8 days (days 4 to 11), cells were cultivated in DMEM/ F12 supplemented with 10% KSR, 1 mM NEAA, 1 mM L-glutamine (Life Technologies), 1% dimethyl sulfoxide (PanReacAppliChem, Darmstadt, Germany), and 100 ng/μl hepatocyte growth factor (PeproTech, Thermo Fisher Scientific).

    Knock-Out:

    Article Title: CRISPR/Cas9-mediated gene correction of Wilson disease H1069Q point mutation in patient-specific induced pluripotent stem cells.
    Article Snippet: .. Briefly, cells were incubated in DMEM/F12 (Gibco, Thermo Fisher Scientific) with increasing KnockOut SR Xenofree medium (KSR; Gibco, Thermo Fisher Scientific) concentrations, enriched with 100 ng/μl recombinant Activin-A (R&D Systems, Minneapolis, MN, USA), 100 ng/μl FGF2 (PeproTech, Thermo Fisher Scientific), and 10 μg/ml recombinant human Wnt-3a (R&D Systems) for 3 days (day 1 to 3). .. For the next 8 days (days 4 to 11), cells were cultivated in DMEM/ F12 supplemented with 10% KSR, 1 mM NEAA, 1 mM L-glutamine (Life Technologies), 1% dimethyl sulfoxide (PanReacAppliChem, Darmstadt, Germany), and 100 ng/μl hepatocyte growth factor (PeproTech, Thermo Fisher Scientific).



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